RT Journal Article
SR Electronic
T1 Genome-wide association study of carbon and nitrogen metabolism in the maize nested association mapping population
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 010785
DO 10.1101/010785
A1 Nengyi Zhang
A1 Yves Gibon
A1 Nicholas Lepak
A1 Pinghua Li
A1 Lauren Dedow
A1 Charles Chen
A1 Yoon-Sup So
A1 Jason Wallace
A1 Karl Kremling
A1 Peter Bradbury
A1 Thomas Brutnell
A1 Mark Stitt
A1 Edward Buckler
YR 2014
UL http://biorxiv.org/content/early/2014/10/28/010785.abstract
AB Carbon (C) and nitrogen (N) metabolism are critical to plant growth and development and at the basis of yield and adaptation. We have applied high throughput metabolite analyses to over 12,000 diverse field grown samples from the maize nested association mapping population. This allowed us to identify natural variation controlling the levels of twelve key C and N metabolites, often with single gene resolution. In addition to expected genes like invertases, critical natural variation was identified in key C4 metabolism genes like carbonic anhydrases and a malate transporter. Unlike prior maize studies, extensive pleiotropy was found for C and N metabolites. This integration of field-derived metabolite data with powerful mapping and genomics resources allows dissection of key metabolic pathways, providing avenues for future genetic improvement.