TY - JOUR T1 - Quantitative mRNA Imaging Throughout the Entire <em>Drosophila</em> Brain JF - bioRxiv DO - 10.1101/096388 SP - 096388 AU - Xi Long AU - Jennifer Colonell AU - Allan M Wong AU - Robert H Singer AU - Timothée Lionnet Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/12/22/096388.abstract N2 - We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample. ER -