PT  - JOURNAL ARTICLE
AU  - Xi Long
AU  - Jennifer Colonell
AU  - Allan M Wong
AU  - Robert H Singer
AU  - Timothée Lionnet
TI  - Quantitative mRNA Imaging Throughout the Entire <em>Drosophila</em> Brain
AID  - 10.1101/096388
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 096388
4099  - http://biorxiv.org/content/early/2016/12/22/096388.short
4100  - http://biorxiv.org/content/early/2016/12/22/096388.full
AB  - We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample.