TY - JOUR T1 - Diatom Centromeres Suggest a Novel Mechanism for Nuclear Gene Acquisition JF - bioRxiv DO - 10.1101/096016 SP - 096016 AU - Rachel E. Diner AU - Chari M. Noddings AU - Nathan C. Lian AU - Anthony K. Kang AU - Jeffrey B. McQuaid AU - Jelena Jablanovic AU - Josh L. Espinoza AU - Ngocquynh A. Nguyen AU - Miguel A. Anzelmatti, Jr. AU - Jakob Jansson AU - Vincent A. Bielinski AU - Bogumil J. Karas AU - Christopher L. Dupont AU - Andrew E. Allen AU - Philip D. Weyman Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/12/22/096016.abstract N2 - Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. Diatom centromere sequences contain low GC content regions and an abundance of long contiguous AT windows, but lack repeats or other conserved sequence features. Native and foreign sequences of similar GC content can maintain episomes and recruit the diatom centromeric histone protein CENP-A, suggesting non-native sequences can also function as diatom centromeres. Thus, simple sequence requirements enable DNA from foreign sources to incorporate into the nuclear genome repertoire as stable extra-chromosomal episomes, revealing a potential mechanism for bacterial and foreign eukaryotic DNA acquisition. ER -