RT Journal Article
SR Electronic
T1 Diatom Centromeres Suggest a Novel Mechanism for Nuclear Gene Acquisition
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 096016
DO 10.1101/096016
A1 Rachel E. Diner
A1 Chari M. Noddings
A1 Nathan C. Lian
A1 Anthony K. Kang
A1 Jeffrey B. McQuaid
A1 Jelena Jablanovic
A1 Josh L. Espinoza
A1 Ngocquynh A. Nguyen
A1 Miguel A. Anzelmatti, Jr.
A1 Jakob Jansson
A1 Vincent A. Bielinski
A1 Bogumil J. Karas
A1 Christopher L. Dupont
A1 Andrew E. Allen
A1 Philip D. Weyman
YR 2016
UL http://biorxiv.org/content/early/2016/12/22/096016.abstract
AB Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. Diatom centromere sequences contain low GC content regions and an abundance of long contiguous AT windows, but lack repeats or other conserved sequence features. Native and foreign sequences of similar GC content can maintain episomes and recruit the diatom centromeric histone protein CENP-A, suggesting non-native sequences can also function as diatom centromeres. Thus, simple sequence requirements enable DNA from foreign sources to incorporate into the nuclear genome repertoire as stable extra-chromosomal episomes, revealing a potential mechanism for bacterial and foreign eukaryotic DNA acquisition.