@article {Diner096016, author = {Rachel E. Diner and Chari M. Noddings and Nathan C. Lian and Anthony K. Kang and Jeffrey B. McQuaid and Jelena Jablanovic and Josh L. Espinoza and Ngocquynh A. Nguyen and Miguel A. Anzelmatti, Jr. and Jakob Jansson and Vincent A. Bielinski and Bogumil J. Karas and Christopher L. Dupont and Andrew E. Allen and Philip D. Weyman}, title = {Diatom Centromeres Suggest a Novel Mechanism for Nuclear Gene Acquisition}, elocation-id = {096016}, year = {2016}, doi = {10.1101/096016}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. Diatom centromere sequences contain low GC content regions and an abundance of long contiguous AT windows, but lack repeats or other conserved sequence features. Native and foreign sequences of similar GC content can maintain episomes and recruit the diatom centromeric histone protein CENP-A, suggesting non-native sequences can also function as diatom centromeres. Thus, simple sequence requirements enable DNA from foreign sources to incorporate into the nuclear genome repertoire as stable extra-chromosomal episomes, revealing a potential mechanism for bacterial and foreign eukaryotic DNA acquisition.}, URL = {https://www.biorxiv.org/content/early/2016/12/22/096016}, eprint = {https://www.biorxiv.org/content/early/2016/12/22/096016.full.pdf}, journal = {bioRxiv} }