@article {Clarke095208, author = {Nicholas I. Clarke and Stephen J. Royle}, title = {FerriTag: A Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy}, elocation-id = {095208}, year = {2016}, doi = {10.1101/095208}, publisher = {Cold Spring Harbor Laboratory}, abstract = {A current challenge is to develop tags to precisely visualize proteins in cells by light and electron microscopy. Here, we introduce FerriTag, a genetically-encoded chemically-inducible tag for correlative light-electron microscopy (CLEM). FerriTag is a fluorescent recombinant electron-dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced heterodimerization. We demonstrate the utility of FerriTag for CLEM by labeling proteins associated with various intracellular structures including mitochondria, plasma membrane, and clathrin-coated pits and vesicles. FerriTagging has a high signal-to-noise ratio and a labeling resolution of 10 {\textpm} 5 nm. We demonstrate how FerriTagging allows nanoscale mapping of protein location relative to a subcellular structure, and use it to detail the distribution of huntingtin-interacting protein 1 related (HIP1R) in clathrin-coated pits.}, URL = {https://www.biorxiv.org/content/early/2016/12/18/095208}, eprint = {https://www.biorxiv.org/content/early/2016/12/18/095208.full.pdf}, journal = {bioRxiv} }