RT Journal Article SR Electronic T1 Long-read, whole-genome shotgun sequence data for five model organisms JF bioRxiv FD Cold Spring Harbor Laboratory SP 008037 DO 10.1101/008037 A1 Kristi E. Kim A1 Paul Peluso A1 Primo Babayan A1 P. Jane Yeadon A1 Charles Yu A1 William W. Fisher A1 Chen-Shan Chin A1 Nicole Rapicavoli A1 David R. Rank A1 Joachim Li A1 David E. A. Catcheside A1 Susan E. Celniker A1 Adam M. Phillippy A1 Casey M. Bergman A1 Jane M. Landolin YR 2014 UL http://biorxiv.org/content/early/2014/10/23/008037.abstract AB Single molecule, real-time (SMRT) sequencing from Pacific Biosciences is increasingly used in many areas of biological research including de novo genome assembly, structural-variant identification, haplotype phasing, mRNA isoform discovery, and base-modification analyses. High-quality, public datasets of SMRT sequences can spur development of analytic tools that can accommodate unique characteristics of SMRT data (long read lengths, lack of GC or amplification bias, and a random error profile leading to high consensus accuracy). In this paper, we describe eight high-coverage SMRT sequence datasets from five organisms (Escherichia coli, Saccharomyces cerevisiae, Neurospora crassa, Arabidopsis thaliana, and Drosophila melanogaster) that have been publicly released to the general scientific community (NCBI Sequence Read Archive ID SRP040522). Data were generated using two sequencing chemistries (P4C2 and P5C3) on the PacBio RS II instrument. The datasets reported here can be used without restriction by the research community to generate whole-genome assemblies, test new algorithms, investigate genome structure and evolution, and identify base modifications in some of the most widely-studied model systems in biological research.