PT  - JOURNAL ARTICLE
AU  - Kristi E. Kim
AU  - Paul Peluso
AU  - Primo Babayan
AU  - P. Jane Yeadon
AU  - Charles Yu
AU  - William W. Fisher
AU  - Chen-Shan Chin
AU  - Nicole Rapicavoli
AU  - David R. Rank
AU  - Joachim Li
AU  - David E. A. Catcheside
AU  - Susan E. Celniker
AU  - Adam M. Phillippy
AU  - Casey M. Bergman
AU  - Jane M. Landolin
TI  - Long-read, whole-genome shotgun sequence data for five model organisms
AID  - 10.1101/008037
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 008037
4099  - http://biorxiv.org/content/early/2014/10/23/008037.short
4100  - http://biorxiv.org/content/early/2014/10/23/008037.full
AB  - Single molecule, real-time (SMRT) sequencing from Pacific Biosciences is increasingly used in many areas of biological research including de novo genome assembly, structural-variant identification, haplotype phasing, mRNA isoform discovery, and base-modification analyses. High-quality, public datasets of SMRT sequences can spur development of analytic tools that can accommodate unique characteristics of SMRT data (long read lengths, lack of GC or amplification bias, and a random error profile leading to high consensus accuracy). In this paper, we describe eight high-coverage SMRT sequence datasets from five organisms (Escherichia coli, Saccharomyces cerevisiae, Neurospora crassa, Arabidopsis thaliana, and Drosophila melanogaster) that have been publicly released to the general scientific community (NCBI Sequence Read Archive ID SRP040522). Data were generated using two sequencing chemistries (P4C2 and P5C3) on the PacBio RS II instrument. The datasets reported here can be used without restriction by the research community to generate whole-genome assemblies, test new algorithms, investigate genome structure and evolution, and identify base modifications in some of the most widely-studied model systems in biological research.