RT Journal Article
SR Electronic
T1 Resolving Biology Beyond the Diffraction Limit with Single-Molecule Localization Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 094839
DO 10.1101/094839
A1 Nafiseh Rafiei
A1 Daniel Nino
A1 Joshua N. Milstein
YR 2016
UL http://biorxiv.org/content/early/2016/12/16/094839.abstract
AB Optical imaging provides a window into the microscopic world, but the level of observable detail is ultimately limited by the wavelength of light being employed. By harnessing the physics of photoswitchable dyes and fluorescent proteins, single-molecule localization microscopy (SMLM) provides a window into the nano-world of biology. This mini-review article provides a short overview of SMLM and discusses some of its prospects for the future.