RT Journal Article
SR Electronic
T1 Dynamically controlled light delivery over large brain volumes through tapered optical fibers
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 094524
DO 10.1101/094524
A1 Ferruccio Pisanello
A1 Gil Mandelbaum
A1 Marco Pisanello
A1 Ian A. Oldenburg
A1 Leonardo Sileo
A1 Jeffrey E. Markowitz
A1 Ralph E. Peterson
A1 Andrea Della Patria
A1 Trevor M. Haynes
A1 Mohamed S. Emara
A1 Barbara Spagnolo
A1 Sandeep R. Datta
A1 Bernardo L. Sabatini
A1 Massimo De Vittorio
YR 2016
UL http://biorxiv.org/content/early/2016/12/15/094524.abstract
AB Optogenetics promises spatiotemporal precise control of neural processes using light. However, the spatial extent of illumination within the brain is difficult to control and cannot be adjusted using standard fiber optics. We demonstrate that optical fibers with tapered tips can be used to illuminate either large brain volumes or dynamically selectable subregions. Remotely adjusting the light input angle to the fiber varies the light-emitting portion of the taper over several millimeters without movement of the implant. We use this mode to activate dorsal versus ventral striatum of individual mice and reveal different effects of each manipulation on motor behavior. Conversely injecting light over the full numerical aperture of the fiber results in light emission from the entire taper surface, achieving broader and more efficient optogenetic activation of neurons when compared to the standard flat-faced fiber stimulation. Thus, tapered fibers permit focal or broad illumination that can be precisely and dynamically matched to experimental needs.