RT Journal Article
SR Electronic
T1 Millstone: Software for Multiplex Microbial Genome Analysis and Engineering
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 086868
DO 10.1101/086868
A1 Daniel B. Goodman
A1 Gleb Kuznetsov
A1 Marc J. Lajoie
A1 Brian W. Ahern
A1 Michael G. Napolitano
A1 Kevin Y. Chen
A1 Changping Chen
A1 George M. Church
YR 2016
UL http://biorxiv.org/content/early/2016/12/15/086868.abstract
AB Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform which automates genotype comparison and visualization for projects with up to hundreds of genomic samples. To enable iterative genome engineering, Millstone allows users to design oligonucleotide libraries and create successive versions of reference genomes. Millstone is open source and easily deployable to a cloud platform, local cluster, or desktop, making it a scalable solution for any lab.