RT Journal Article SR Electronic T1 Deconstructing isolation-by-distance: the genomic consequences of limited dispersal JF bioRxiv FD Cold Spring Harbor Laboratory SP 093989 DO 10.1101/093989 A1 Stepfanie M. Aguillon A1 John W. Fitzpatrick A1 Reed Bowman A1 Stephan J. Schoech A1 Andrew G. Clark A1 Graham Coop A1 Nancy Chen YR 2016 UL http://biorxiv.org/content/early/2016/12/14/093989.abstract AB Geographically limited dispersal can shape genetic population structure and result in a correlation between genetic and geographic distance, commonly called isolation-by-distance. Despite the prevalence of isolation-by-distance in nature, to date few studies have empirically demonstrated the processes by which it is generated within a pedigreed and genotyped population. Intensive, long-term demographic studies and exhaustive genomic surveys in the Florida Scrub-Jay (Aphelocoma coerulescens) provide an excellent opportunity to investigate the influence of dispersal on genetic structure. Here, we use a panel of genome-wide SNPs and extensive pedigree information to explore the role of limited dispersal in shaping patterns of isolation-by-distance in both sexes, and at an exceedingly fine spatial scale (within ∼10 km). Isolation-by-distance patterns were stronger in male-male and male-female comparisons than in female-female comparisons, consistent with observed differences in dispersal propensity between the sexes. Using the pedigree, we demonstrated how various genealogical relationships contribute to fine-scale isolation-by-distance. Simulations using field-observed distributions of male and female natal dispersal distances showed good agreement with the distribution of geographic distances between breeding individuals of different pedigree relationship classes. Furthermore, we can accurately reconstruct observed isolation-by-distance patterns in autosomal and Zlinked SNPs using coalescent simulations parameterized by the observed dispersal curve, population density, and immigration rate. Therefore, patterns of fine-scale isolation-by-distance can be well understood as a result of limited dispersal over contemporary timescales in the Florida Scrub-Jay.