RT Journal Article SR Electronic T1 Insights on the importance of salinity from the first cultured freshwater SAR11 (LD12) representative JF bioRxiv FD Cold Spring Harbor Laboratory SP 093567 DO 10.1101/093567 A1 Michael W. Henson A1 V. Celeste Lanclos A1 J. Cameron Thrash YR 2016 UL http://biorxiv.org/content/early/2016/12/13/093567.abstract AB Bacterioplankton of the SAR11 clade dominate aquatic ecosystems but knowledge of their freshwater members remains limited due to a lack of cultured representatives. Here, we report the first isolate from the freshwater SAR11 subclade lllb, a.k.a. LD12, obtained from surface waters in Lake Borgne, a lagoon on the southeastern coast of Louisiana. Consistent with pervasive ecological data, strain LSUCC0530 is highly restricted to growth at low salinities. Comparison of its distribution with the sister clade subclade llla, however, suggests that niche differentiation between sister taxa in coastal environments may be driven by more than salinity alone.