PT - JOURNAL ARTICLE AU - Murray Patterson AU - Thomas Bernard AU - Daniel Kahn TI - Correlated Evolution of Metabolic Functions over the Tree of Life AID - 10.1101/093591 DP - 2016 Jan 01 TA - bioRxiv PG - 093591 4099 - http://biorxiv.org/content/early/2016/12/13/093591.short 4100 - http://biorxiv.org/content/early/2016/12/13/093591.full AB - We are interested in the structure and evolution of metabolism in order to better understand its complexity. We study metabolic functions in 1459 species within which several hundreds of thousands of families of homologous genes have been identified [17]. Given a protein sequence, PRIAM search [5] delivers probabilities of the presence of several thousand enzymes (ECs). This allows us to infer reaction sets and to construct a metabolic network for an organism, given its set of sequences.We then propagate these ECs to the ancestral nodes of the species tree using maximimum likelihood methods. These evolutionary scenarios are systematically compared using pairwise mutual information. We identify co-evolving enzyme sets from the graph of these relationships using community detection algorithms [1,3]. This sheds light on the structure of the metabolic networks in terms of co-evolving metabolic modules. These modules are also interpreted from a functional perspective using stoichiometric models of metabolic networks.