RT Journal Article SR Electronic T1 A simple method for constructing magnetic Escherichia coli JF bioRxiv FD Cold Spring Harbor Laboratory SP 010249 DO 10.1101/010249 A1 Mengyi Sun A1 Yong-jun Lu YR 2014 UL http://biorxiv.org/content/early/2014/10/10/010249.abstract AB Magnetic force can serve as an ideal way to control the spatial behavior of microorganisms, because of its flexibility and penetrability. By incubation with the biocompatible compound, ammonium ferric citrate, as an iron source, we magnetized Escherichia coli, the most programmable chassis in synthetic biology. To enhance the magnetization efficiency, the ferritin protein, FtnA, from E. coli was cloned and overexpressed in strain BL21(DE3). The magnetization effect was observed within 30 min after harvest of bacteria, and the concentration of ammonium ferric acid used could be as low as 0.5 mM. Using different shapes of magnetic fields, different patterns could be generated easily. Our method may set up the foundation for a rational design of spatial structure of cell communities, which is important for their actual application.