PT  - JOURNAL ARTICLE
AU  - Seungsoo Kim
AU  - Ivan Liachko
AU  - Donna G Brickner
AU  - Kate Cook
AU  - William S Noble
AU  - Jason H Brickner
AU  - Jay Shendure
AU  - Maitreya J Dunham
TI  - The dynamic three-dimensional organization of the diploid yeast genome
AID  - 10.1101/091827
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 091827
4099  - http://biorxiv.org/content/early/2016/12/05/091827.short
4100  - http://biorxiv.org/content/early/2016/12/05/091827.full
AB  - The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. Even in this well-studied model, it is unclear how homolog pairing in diploids and environment-induced gene relocalization influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation, we observe significant homolog proximity that increased in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1 alleles specifically under galactose induction and saturated growth, mediated by association with nuclear pore complexes at the nuclear periphery. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.