RT Journal Article SR Electronic T1 Reprogramming protein kinase substrate specificity through synthetic mutations JF bioRxiv FD Cold Spring Harbor Laboratory SP 091892 DO 10.1101/091892 A1 Joshua M. Lubner A1 George M. Church A1 Michael F. Chou A1 Daniel Schwartz YR 2016 UL http://biorxiv.org/content/early/2016/12/05/091892.abstract AB Protein kinase specificity is largely imparted through substrate binding pocket motifs. Missense mutations in these regions are frequently associated with human disease, and in some cases can alter substrate specificity. However, current efforts at decoding the influence of mutations on substrate specificity have been focused on disease-associated mutations. Here, we adapted the Proteomic Peptide Library (ProPeL) approach for determining kinase specificity to the task of exploring structure-function relationships in kinase specificity by interrogating the effects of synthetic mutation. We established a specificity model for the wild-type DYRK1A kinase with unprecedented resolution. Using existing crystallographic and sequence homology data, we rationally designed mutations that precisely reprogrammed the DYRK1A kinase at the P+1 position to mimic the substrate preferences of a related kinase, CK II. This study illustrates a new synthetic biological approach to reprogram kinase specificity by design, and a powerful new paradigm to investigate structure-function relationships underpinning kinase substrate specificity.