RT Journal Article SR Electronic T1 Sexually satiated male Drosophila melanogaster uses gustatory-to-neuropeptide integrative circuits to reduce time investment for mating JF bioRxiv FD Cold Spring Harbor Laboratory SP 088724 DO 10.1101/088724 A1 Woo Jae Kim A1 Seung Gee Lee A1 Anne-Christine Auge A1 Lily Yeh Jan A1 Yuh Nung Jan YR 2016 UL http://biorxiv.org/content/early/2016/11/22/088724.abstract AB Males have a finite resource for reproduction; they rely on a ‘time investment strategy’ to maximize reproductive success. Here we report a novel behavioral plasticity whereby male fruit flies exhibit a shortened mating duration when sexually satiated, which we named ‘Shorter-Mating-Duration (SMD)’. SMD requires the sexually dimorphic Gr5a-positive neurons for detecting female body pheromones, and can be induced by gustatory stimuli. The memory circuitry within the ellipsoid body (EB) and mushroom body (MB) brain regions is crucial for SMD, which depends on the circadian clock genes Clock and cycle, but not timeless or period. SMD also relies on signaling via the neuropeptide sNPF, but not PDF or NPF. Sexual experience modifies the neuronal activity of a subset of sNPF-positive neurons involved in neuropeptide signaling, which modulates SMD. Thus, our study delineates the molecular and cellular basis for SMD – a plastic social behavior that serves as a model system to study how the brain switches the internal states between sexual drive and satiety.