RT Journal Article SR Electronic T1 Mycobacterium ulcerans low infectious dose and atypical mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer JF bioRxiv FD Cold Spring Harbor Laboratory SP 071753 DO 10.1101/071753 A1 John R. Wallace A1 Kirstie M. Mangas A1 Jessica L. Porter A1 Renee Marcsisin A1 Sacha J. Pidot A1 Brian Howden A1 Till F. Omansen A1 Weiguang Zeng A1 Jason K. Axford A1 Paul D. R. Johnson A1 Timothy P. Stinear YR 2016 UL http://biorxiv.org/content/early/2016/11/21/071753.abstract AB Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 11 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. Eighteen of 20 mice subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. We observed a low infectious dose-50 of four colony-forming units and a median incubation time of 12 weeks, consistent with data from human infections. We have uncovered a highly efficient and biologically plausible atypical transmission mode of BU via natural or anthropogenic skin punctures.Author summary Buruli ulcer is a neglected tropical disease caused by infection with Mycobacterium ulcerans. Unfortunately, how people contract this disease is not well understood. Here we show for the first time using experimental infections in mice that a very low dose of M. ulcerans delivered beneath the skin by a minor injury caused by a blood-feeding insect (mosquito) or a needle puncture is sufficient to cause Buruli ulcer. This research provides important laboratory evidence to advance our understanding of Buruli ulcer disease transmission.