RT Journal Article
SR Electronic
T1 Immunological Patterns from Four Melioidosis Cases: Constant and Variable Protein Antigens
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 082057
DO 10.1101/082057
A1 Jinhee Yi
A1 Kelsey Herring
A1 Timothy C. Sanchez
A1 Srinivas Iyer
A1 Joshua K. Stone
A1 Judy Lee
A1 Mark Mayo
A1 Bart J. Currie
A1 Apichai Tuanyok
A1 Paul Keim
YR 2016
UL http://biorxiv.org/content/early/2016/11/20/082057.abstract
AB Burkholderia pseudomallei is the causative agent of the melioidosis and is endemic to Southeast Asia and northern Australia. There is no available vaccine and accurate diagnosis is difficult, time-consuming and labor intensive. Early diagnosis is an important part of successful treatment and current serological tests are inadequate and based upon multiple antigens. Identifying specific immunogenic proteins which are highly seroreactive may yield potential diagnostic targets for detecting antibodies and antigens specific to melioidosis. We have used 2D gel electrophoresis and Western blotting analysis to analyze protein antigenicity of whole cell lysates extracted from four B. pseudomallei strains and the sera from the specific infected humans. We found a total of 135 immunogenic proteins, 62 of which we were able to identify to a specific gene by mass-spectrometry. Results from the Western blotting of each strain’s proteins and the corresponding patient serum reveal between 30 – 40% serum x strain specific immunogenic proteins. In most cases, these differences exist despite the fact that the genes encoding these proteins were present among all four B. pseudomallei strains. Eight particular proteins were immunogenic in all four strain x serum combinations and could represent novel diagnostic and vaccine subunit targets.