RT Journal Article SR Electronic T1 Visualizing adenosine to inosine RNA editing in single mammalian cells JF bioRxiv FD Cold Spring Harbor Laboratory SP 088146 DO 10.1101/088146 A1 Ian A. Mellis A1 Rohit K. Gupte A1 Arjun Raj A1 Sara H. Rouhanifard YR 2016 UL http://biorxiv.org/content/early/2016/11/16/088146.abstract AB Conversion of adenosine bases to inosine in RNA is a frequent type of RNA editing, but important details about its biology, including subcellular localization, remain unknown due to a lack of imaging tools. We developed an RNA FISH strategy we called inoFISH that enables us to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. Applying this tool to three edited transcripts (GRIA2, EIF2AK2 and NUP43), we found that editing of these transcripts is not correlated with nuclear localization nor paraspeckle association, and that NUP43 exhibits constant editing rates between single cells while the rates for GRIA2 vary.