RT Journal Article
SR Electronic
T1 Cobalt enrichment in anaerobic microbial cocultures revealed by synchrotron X-ray fluorescence imaging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 087585
DO 10.1101/087585
A1 Jennifer B. Glass
A1 Si Chen
A1 Katherine S. Dawson
A1 Damian R. Horton
A1 Stefan Vogt
A1 Ellery D. Ingall
A1 Benjamin S. Twining
A1 Victoria J. Orphan
YR 2016
UL http://biorxiv.org/content/early/2016/11/14/087585.abstract
AB This study examined iron, cobalt, nickel, copper, and zinc content of a model sulfate-reducing bacterium and methanogenic archaeon in mono- vs. coculture. Inductively coupled plasma mass spectrometry and synchrotron x-ray fluorescence microscopy were used to compare elemental content of bulk vs. single cells. Cocultures contained more cellular cobalt than monocultures as well as distinct nanoparticulate zinc- and cobalt/copper-sulfides. This study provides the first evidence that microbes have different metal quotas in mono- vs. coculture, and that cocultures grown in micromolar metal concentrations precipitate different metal sulfide minerals than previous studies of sulfate-reducing bacteria grown at millimolar metal concentrations.