PT  - JOURNAL ARTICLE
AU  - Jennifer B. Glass
AU  - Si Chen
AU  - Katherine S. Dawson
AU  - Damian R. Horton
AU  - Stefan Vogt
AU  - Ellery D. Ingall
AU  - Benjamin S. Twining
AU  - Victoria J. Orphan
TI  - Cobalt enrichment in anaerobic microbial cocultures revealed by synchrotron X-ray fluorescence imaging
AID  - 10.1101/087585
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 087585
4099  - http://biorxiv.org/content/early/2016/11/14/087585.short
4100  - http://biorxiv.org/content/early/2016/11/14/087585.full
AB  - This study examined iron, cobalt, nickel, copper, and zinc content of a model sulfate-reducing bacterium and methanogenic archaeon in mono- vs. coculture. Inductively coupled plasma mass spectrometry and synchrotron x-ray fluorescence microscopy were used to compare elemental content of bulk vs. single cells. Cocultures contained more cellular cobalt than monocultures as well as distinct nanoparticulate zinc- and cobalt/copper-sulfides. This study provides the first evidence that microbes have different metal quotas in mono- vs. coculture, and that cocultures grown in micromolar metal concentrations precipitate different metal sulfide minerals than previous studies of sulfate-reducing bacteria grown at millimolar metal concentrations.