%0 Journal Article %A Justin R. Klesmith %A Sarah Thorwall %A Timothy A. Whitehead %T Interpreting deep mutational scanning data resulting from selections on solid media %D 2016 %R 10.1101/087072 %J bioRxiv %P 087072 %X Deep mutational scanning is now used in directed evolution experiments to quantify the change in frequency of a cellular variant in a mixed population. A key concern is the extent to which the enrichment of a variant in a population corresponds to a fitness metric like relative growth rate or survival percentage. We present here analytical equations converting the enrichment of a variant to fitness metrics for plate-based selections. Using isogenic and mixed cultures we show that growth rates and survival percentages correlate for antibiotic plate-based selections. These results are important for proper interpretation of data resulting from deep sequencing. %U https://www.biorxiv.org/content/biorxiv/early/2016/11/10/087072.full.pdf