TY - JOUR T1 - RNA-seq library preparation from single pancreatic acinar cells JF - bioRxiv DO - 10.1101/085696 SP - 085696 AU - Damian Wollny AU - Sheng Zhao AU - Ana Martin-Villalba Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/11/04/085696.abstract N2 - Single cell RNA sequencing technology has emerged as a promising tool to uncover previously neglected cellular heterogeneity. Multiple methods and protocols have been developed to apply single cell sequencing to different cell types from various organs. However, library preparation for RNA sequencing remains challenging for cell types with high RNAse content due to rapid degradation of endogenous RNA molecules upon cell lysis. To this end, we developed a protocol based on the SMART-seq2 technology for single cell RNA sequencing of pancreatic acinar cells, the cell type with one of the highest ribonuclease concentration measured to date. This protocol reliably produces high quality libraries from single acinar cells reaching a total of 5x106 reads / cell and ∼ 80% transcript mapping rate with no detectable 3´end bias. Thus, our protocol makes single cell transcriptomics accessible to cell type with very high RNAse content. ER -