PT - JOURNAL ARTICLE AU - Matthew D. MacManes AU - Michael B. Eisen TI - Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse <em>Peromyscus eremicus</em> AID - 10.1101/009134 DP - 2014 Jan 01 TA - bioRxiv PG - 009134 4099 - http://biorxiv.org/content/early/2014/09/15/009134.short 4100 - http://biorxiv.org/content/early/2014/09/15/009134.full AB - As a direct result of intense heat and aridity, deserts are thought to be among the most harsh of environments, particularly for their mammalian inhabitants. Given that osmoregulation can be challenging for these animals, with failure resulting in death, strong selection should be observed on genes related to the maintenance of water and solute balance. One such animal, Peromyscus eremicus, is native to the desert regions of the southwest United States and may live its entire life without oral fluid intake. As a first step toward understanding the genetics that underlie this phenotype, we present a characterization of the P. eremicus transcriptome. We assay four tissues (kidney, liver, brain, testes) from a single individual and supplement this with population level renal transcriptome sequencing from 15 additional animals. We identified a set of transcripts undergoing both purifying and balancing selection based on estimates of Tajima’s D. In addition, we used the branch-site test to identify a transcript -Slc2a9, likely related to desert osmoregulation – undergoing enhanced selection in P. eremicus relative to a set of related non-desert rodents.