RT Journal Article
SR Electronic
T1 Pooled CRISPR screening with single-cell transcriptome read-out
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 083774
DO 10.1101/083774
A1 Paul Datlinger
A1 Christian Schmidl
A1 André F Rendeiro
A1 Peter Traxler
A1 Johanna Klughammer
A1 Linda Schuster
A1 Christoph Bock
YR 2016
UL http://biorxiv.org/content/early/2016/10/27/083774.abstract
AB CRISPR-based genetic screens have revolutionized the search for new gene functions and biological mechanisms. However, widely used pooled screens are limited to simple read-outs of cell proliferation or the production of a selectable marker protein. Arrayed screens allow for more complex molecular read-outs such as transcriptome profiling, but they provide much lower throughput. Here we demonstrate CRISPR genome editing together with single-cell RNA sequencing as a new screening paradigm that combines key advantages of pooled and arrayed screens. This approach allowed us to link guide-RNA expression to the associated transcriptome responses in thousands of single cells using a straightforward and broadly applicable screening workflow.