TY - JOUR T1 - UNC-5 (UNC5) Regulates the Length and Number of Processes that <em>Caenorhabditis elegans</em> Neurons Can Develop JF - bioRxiv DO - 10.1101/083436 SP - 083436 AU - Gerard Limerick AU - Xia Tang AU - Won Suk Lee AU - Ahmed Mohamed AU - Aseel Al-Aamiri AU - William G. Wadsworth Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/25/083436.abstract N2 - Neurons extend processes that vary in number, length, and direction of outgrowth. Extracellular molecules act as cues to regulate this patterning. In Caenorhabditis elegans, neurons respond to the UNC-6 (netrin) cue via UNC-5 (UNC5) and UNC-40 (DCC) receptors. Here we present evidence that UNC-5 regulates the length and number of processes that neurons develop. Genetic analysis suggests UNC-5 functions with UNC-6 and EGL-20 (wnt), the SAX-3 (Robo) receptor, and the cytoplasmic proteins UNC-53 (NAV2), MIG-15 (NIK kinase), and MADD-2 (TRIM) to regulate the asymmetric localization of UNC-40 to a surface of the neuron. We have postulated that UNC-40 polarization is self-organizing and that the surface to which UNC-40 localizes and mediates outgrowth is stochastically determined. At any instance of time, there is a probability that UNC-40-mediated outgrowth will occur in a specific direction. We find that UNC-5 activity reduces the degree to which the direction of outgrowth fluctuations over time. Random walk modeling predicts that by decreasing the fluctuation UNC-5 activity increases the mean-squared distance that outgrowth movement could covered over a given time. This suggests that UNC-5 activity creates outgrowth patterns by varying the rate of outgrowth along a surface of the neuron. ER -