PT  - JOURNAL ARTICLE
AU  - Elena Colangelo
AU  - Qiubo Chen
AU  - Adam M. Davidson
AU  - David Paramelle
AU  - Michael B. Sullivan
AU  - Martin Volk
AU  - Raphaël Lévy
TI  - Experimental and Computational Investigation of the Structure of Peptide Monolayers on Gold Nanoparticles
AID  - 10.1101/083204
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 083204
4099  - http://biorxiv.org/content/early/2016/10/25/083204.short
4100  - http://biorxiv.org/content/early/2016/10/25/083204.full
AB  - The self-assembly and self-organization of small molecules at the surface of nanoparticles constitute a potential route towards the preparation of advanced protein-like nanosystems. However, their structural characterization, critical to the design of bio-nanomaterials with well-defined biophysical and biochemical properties, remains highly challenging. Here, a computational model for peptide-capped gold nanoparticles is developed using experimentally characterized CALNN-and CFGAILSS-capped gold nanoparticles as a benchmark. The structure of CALNN and CFGAILSS monolayers is investigated by both structural biology techniques and molecular dynamics simulations. The calculations reproduce the experimentally observed dependence of the monolayer secondary structure on peptide capping density and on nanoparticle size, thus giving us confidence in the model. Furthermore, the computational results reveal a number of new features of peptide-capped monolayers, including the importance of sulfur movement for the formation of secondary structure motifs, the presence of water close to the gold surface even in tightly packed peptide monolayers, and the existence of extended 2D parallel β-sheet domains in CFGAILSS monolayers. The model developed here provides a predictive tool that may assist in the design of further bio-nanomaterials.