TY - JOUR T1 - Extremely fast and incredibly close: co-transcriptional splicing in budding yeast JF - bioRxiv DO - 10.1101/083170 SP - 083170 AU - Edward W.J. Wallace AU - Jean D. Beggs Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/25/083170.abstract N2 - RNA splicing, an essential part of eukaryotic pre-messenger RNA processing, can be simultaneous with transcription by RNA polymerase II. Here, we compare and review independent next-generation sequencing methods that quantify co-transcriptional splicing in budding yeast. Splicing in yeast is fast, taking place within seconds of intron transcription, while polymerase is within a few dozens of nucleotides of the 3’ splice site. Ribosomal protein mRNAs are spliced particularly fast and co-transcriptionally. Intron-mediated regulation of some genes is also likely to be co-transcriptional. We suggest that intermediates of the splicing reaction, missing from current datasets, may hold key information about splicing kinetics.TrendsIndependent next-generation sequencing methods quantify co-transcriptional splicing in budding yeastRibosomal protein mRNAs are spliced particularly fast and co-transcriptionallyIntron-mediated regulation of DBP2 and RPS9A is likely co-transcriptionalSplicing intermediates, missing from current datasets, may hold key information ER -