@article {Wallace083170, author = {Edward W.J. Wallace and Jean D. Beggs}, title = {Extremely fast and incredibly close: co-transcriptional splicing in budding yeast}, elocation-id = {083170}, year = {2016}, doi = {10.1101/083170}, publisher = {Cold Spring Harbor Laboratory}, abstract = {RNA splicing, an essential part of eukaryotic pre-messenger RNA processing, can be simultaneous with transcription by RNA polymerase II. Here, we compare and review independent next-generation sequencing methods that quantify co-transcriptional splicing in budding yeast. Splicing in yeast is fast, taking place within seconds of intron transcription, while polymerase is within a few dozens of nucleotides of the 3{\textquoteright} splice site. Ribosomal protein mRNAs are spliced particularly fast and co-transcriptionally. Intron-mediated regulation of some genes is also likely to be co-transcriptional. We suggest that intermediates of the splicing reaction, missing from current datasets, may hold key information about splicing kinetics.TrendsIndependent next-generation sequencing methods quantify co-transcriptional splicing in budding yeastRibosomal protein mRNAs are spliced particularly fast and co-transcriptionallyIntron-mediated regulation of DBP2 and RPS9A is likely co-transcriptionalSplicing intermediates, missing from current datasets, may hold key information}, URL = {https://www.biorxiv.org/content/early/2016/10/25/083170}, eprint = {https://www.biorxiv.org/content/early/2016/10/25/083170.full.pdf}, journal = {bioRxiv} }