TY - JOUR T1 - Casein Kinase II is Required for Proper Cell Division and Acts as a Negative Regulator of Centrosome Duplication in <em>C. elegans</em> Embryos JF - bioRxiv DO - 10.1101/083378 SP - 083378 AU - Jeffrey C. Medley AU - Megan M. Kabara AU - Michael D. Stubenvoll AU - Lauren E. DeMeyer AU - Mi Hye Song Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/25/083378.abstract N2 - Centrosomes are the primary microtubule-organizing centers that orchestrate microtubule dynamics during the cell cycle. The correct number of centrosomes is pivotal for establishing bipolar mitotic spindles that ensure accurate segregation of chromosomes. Thus, centrioles must duplicate once per cell cycle, one daughter per mother centriole, the process of which requires highly coordinated actions among core factors and modulators. Protein phosphorylation is shown to regulate the stability, localization and activity of centrosome proteins. Here, we report the function of Casein Kinase II (CK2) in early C. elegans embryos. The catalytic subunit (KIN-3/CK2α) of CK2 localizes to nuclei, centrosomes and midbodies. Inactivating CK2 leads to cell division defects, including chromosome missegregation, cytokinesis failure and aberrant centrosome behavior. Furthermore, depletion or inhibiting kinase activity of CK2 results in elevated ZYG-1 levels at centrosomes, restoring centrosome duplication and embryonic viability to zyg-1 mutants. Our data suggest that CK2 functions in cell division and negatively regulates centrosome duplication in a kinase-dependent manner.Summary statement The conserved protein kinase CK2 negatively regulates centrosome assembly and is required for proper cell cycle progression and cytokinesis in early C. elegans embryos.Abbreviations(CK2)Casein Kinase II(TBB)4,5,6,7-Tetrabromobenotriazole(DMSO)Dimethyl sulfoxide(PP2A)Protein Phosphatase 2A(PCM)Pericentriolar Material(IP)Pericentriolar Material(PH)Pleckstrin Homology ER -