RT Journal Article
SR Electronic
T1 Systematic Imaging Reveals Features of Localized mRNAs and Their Changing Subcellular Destinations in Development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 008938
DO 10.1101/008938
A1 Helena Jambor
A1 Vineeth Surendranath
A1 Alex T. Kalinka
A1 Pavel Mejstrik
A1 Stephan Saalfeld
A1 Pavel Tomancak
YR 2014
UL http://biorxiv.org/content/early/2014/09/09/008938.abstract
AB The asymmetric distribution of cytoplasmic components by mRNA localization is critical for eukaryotic cells and affects large numbers of transcripts. How such global subcellular localization of mRNAs is regulated is still unknown. We combined transcriptomics and systematic imaging to determine tissue-specific expression and subcellular localizations of 5862 mRNAs during Drosophila oogenesis. While the transcriptome is stable and alternative splicing and polyadenylation is rare, cytoplasmic localization of mRNAs is widespread. Localized mRNAs have distinct gene features and diverge in expression level, 3’UTR length and sequence conservation. We show that intracellular localization of mRNAs depends on an intact microtubule cytoskeleton and that specifically the posterior enrichment requires the localization of oskar mRNA to the posterior cortex. Using cross-tissue comparison we revealed that the localization landscape differs substantially between epithelial, germline and embryonic cells and the localization status of mRNAs also changes considerably within the oocyte over the course of oogenesis.