TY - JOUR T1 - Systematic Imaging Reveals Features of Localized mRNAs and Their Changing Subcellular Destinations in Development JF - bioRxiv DO - 10.1101/008938 SP - 008938 AU - Helena Jambor AU - Vineeth Surendranath AU - Alex T. Kalinka AU - Pavel Mejstrik AU - Stephan Saalfeld AU - Pavel Tomancak Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/09/09/008938.abstract N2 - The asymmetric distribution of cytoplasmic components by mRNA localization is critical for eukaryotic cells and affects large numbers of transcripts. How such global subcellular localization of mRNAs is regulated is still unknown. We combined transcriptomics and systematic imaging to determine tissue-specific expression and subcellular localizations of 5862 mRNAs during Drosophila oogenesis. While the transcriptome is stable and alternative splicing and polyadenylation is rare, cytoplasmic localization of mRNAs is widespread. Localized mRNAs have distinct gene features and diverge in expression level, 3’UTR length and sequence conservation. We show that intracellular localization of mRNAs depends on an intact microtubule cytoskeleton and that specifically the posterior enrichment requires the localization of oskar mRNA to the posterior cortex. Using cross-tissue comparison we revealed that the localization landscape differs substantially between epithelial, germline and embryonic cells and the localization status of mRNAs also changes considerably within the oocyte over the course of oogenesis. ER -