TY - JOUR T1 - Genome-wide characterization of RNA editing in chicken: lack of evidence for non-A-to-I events JF - bioRxiv DO - 10.1101/008912 SP - 008912 AU - Laure Frésard AU - Sophie Leroux AU - Pierre-François Roux AU - Christophe Klopp AU - Stéphane Fabre AU - Diane Esquerré AU - Patrice Dehais AU - Anis Djari AU - David Gourichon AU - Sandrine Lagarrigue AU - Frédérique Pitel Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/09/09/008912.abstract N2 - RNA editing corresponds to a post-transcriptional nucleotide change in the RNA sequence, creating an alternative nucleotide, not present in the DNA sequence. This leads to a diversification of transcription products with potential functional consequences. Two nucleotide substitutions are mainly described in animals, from adenosine to inosine (A-to-I) and from cytidine to uridine (C-to-U). This phenomenon is more and more described in mammals, notably since the availability of next generation sequencing technologies allowing a whole genome screening of RNA-DNA differences. The number of studies recording RNA editing in other vertebrates like chicken are still limited. We chose to use high throughput sequencing technologies to search for RNA editing in chicken, to understand to what extent this phenomenon is conserved in vertebrates.We performed RNA and DNA sequencing from 8 embryos. Being aware of common pitfalls inherent to sequence analyses leading to false positive discovery, we stringently filtered our datasets and found less than 40 reliable candidates. Conservation of particular sites of RNA editing was attested by the presence of 3 edited sites previously detected in mammals. We then characterized editing levels for selected candidates in several tissues and at different time points, from 4.5 days of embryonic development to adults, and observed a clear tissue-specificity and a gradual editing level increase with time.By characterizing the RNA editing landscape in chicken, our results highlight the extent of evolutionary conservation of this phenomenon within vertebrates, and provide support of an absence of non A-to-I events from the chicken transcriptome. ER -