@article {Michieletto058933, author = {D. Michieletto and E. Orlandini and D. Marenduzzo}, title = {A Polymer Model with Epigenetic Recolouring Reveals a Pathway for the de novo Establishment and 3D organisation of Chromatin Domains}, elocation-id = {058933}, year = {2016}, doi = {10.1101/058933}, publisher = {Cold Spring Harbor Laboratory}, abstract = {One of the most important problems in development is how epigenetic domains can be first established, and then maintained, within cells. To address this question, we propose a framework which couples 3D chromatin folding dynamics, to a {\textquotedblleft}recolouring{\textquotedblright} process modeling the writing of epigenetic marks. Because many intra-chromatin interactions are mediated by bridging proteins, we consider a {\textquotedblleft}two-state{\textquotedblright} model with self-attractive interactions between two epigenetic marks which are alike (either active or inactive). This model displays a first-order-like transition between a swollen, epigenetically disordered, phase, and a compact, epigenetically coherent, chromatin globule. If the self-attraction strength exceeds a threshold, the chromatin dynamics becomes glassy, and the corresponding interaction network freezes. By modifying the epigenetic read-write process according to more biologically-inspired assumptions, our polymer model with recolouring recapitulates the ultrasensitive response of epigenetic switches to perturbations, and accounts for long-lived multi-domain conformations, strikingly similar to the topologically-associating-domains observed in eukaryotic chromosomes.}, URL = {https://www.biorxiv.org/content/early/2016/10/23/058933}, eprint = {https://www.biorxiv.org/content/early/2016/10/23/058933.full.pdf}, journal = {bioRxiv} }