RT Journal Article
SR Electronic
T1 Potent optogenetic inhibition of behavior with anion channelrhodopsins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 082255
DO 10.1101/082255
A1 Farhan Mohammad
A1 James C. Stewart
A1 Stanislav Ott
A1 Katarina Chlebikova
A1 Jia Yi Chua
A1 Tong-Wey Koh
A1 Joses Ho
A1 Adam Claridge-Chang
YR 2016
UL http://biorxiv.org/content/early/2016/10/20/082255.abstract
AB Optogenetics employs light exposure to manipulate physiology in genetically modified organisms. There are abundant tools for optogenetic excitation of neuronal activity, but the limitations of current activity photo-inhibitors present an obstacle to demonstrating the necessity of specific neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit a range of systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, demonstrating their broad utility to the circuit analysis of behavior.