RT Journal Article SR Electronic T1 Kinematic analysis of cell lineage reveals coherent and robust mechanical deformation patterns in zebrafish gastrulation JF bioRxiv FD Cold Spring Harbor Laboratory SP 054353 DO 10.1101/054353 A1 David Pastor-Escuredo A1 Benoît Lombardot A1 Thierry Savy A1 Adeline Boyreau A1 Jose M. Goicolea A1 Andrés Santos A1 Paul Bourgine A1 Juan C. del Álamo A1 Nadine Peyriéras A1 María J. Ledesma-Carbayo YR 2016 UL http://biorxiv.org/content/early/2016/10/19/054353.abstract AB Digital cell lineages reconstructed from 3D+time imaging data provide unique information to unveil mechanical cues and their role in morphogenetic processes. Our methodology based on a kinematic analysis of cell lineage data reveals deformation patterns and quantitative morphogenetic landmarks for a new type of developmental table. The characteristic spatial and temporal length scales of mechanical deformation patterns derived from a continuous approximation of cell displacements indicate a compressible fluid-like behavior of zebrafish gastrulating tissues. The instantaneous deformation rate at the mesoscopic level of the cell’s neighborhood is spatially and temporally heterogeneous. The robustness of mechanical patterns results from their cumulative history along cell trajectories. Unsupervised classification of mechanical descriptor profiles was used to assess the homogeneity of biomechanical cues in cell populations. Further clustering of cell trajectories according to their cumulative mesoscopic biomechanical history during gastrulation revealed ordered and coherent spatiotemporal patterns comparable to that of the embryonic fate map.