TY - JOUR T1 - Computational study of the impacts of mechanical and physical cell properties on mitotic cell rounding in developing epithelia JF - bioRxiv DO - 10.1101/037820 SP - 037820 AU - Ali Nematbakhsh AU - Wenzhao Sun AU - Pavel A. Brodskiy AU - Aboutaleb Amiri AU - Cody Narciso AU - Zhiliang Xu AU - Jeremiah J. Zartman AU - Mark Alber Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/17/037820.abstract N2 - Mitotic rounding (MR) during cell division is critical for the robust segregation of chromosomes into daughter cells and is frequently perturbed in cancerous cells. MR has been studied extensively in individual cultured cells, but the physical mechanisms regulating MR in intact tissues are still poorly understood. A cell undergoes mitotic rounding by simultaneously reducing adhesion with its neighbors, increasing actomyosin contraction around the cortex, and increasing the osmotic pressure of the cytoplasm. Whether these changes are purely additive, synergistic or impact separate aspects of MR is not clear. Specific modulation of these processes in dividing cells within a tissue is experimentally challenging, because of off-target effects and the difficulty of targeting only dividing cells. In this study, we analyze MR in epithelial cells by using a newly developed multi-scale, cell-based computational model that is calibrated using experimental observations from a model system of epithelial tissue growth, the Drosophila wing imaginal disc. The model simulations predict that increase in apical surface area of mitotic cells is solely driven by increasing cytoplasmic pressure. MR however is not achieved within biological constraints unless all three properties (cell-cell adhesion, cortical stiffness and pressure) are simultaneously regulated by the cell. The new multi-scale model is computationally implemented using a parallelization algorithm on a cluster of graphic processing units (GPUs) to make simulations of tissues with a large number of cells feasible. The model is extensible to investigate a wide range of cellular phenomena at the tissue scale.Author Summary Mitotic rounding (MR) during cell division is critical for the robust segregation of chromosomes into daughter cells and is frequently perturbed in cancerous cells. MR has been studied extensively in individual cultured cells, but the physical mechanisms regulating MR in intact tissues are still poorly understood. The newly developed computational model Epi-scale enables one to produce new hypotheses about the underlying biophysical mechanisms governing MR of epithelial cells within the developing tissue micro-environment. In particular, our simulations results predict that robust mitotic rounding requires co-current changes in cell-cell adhesion, cortical stiffness and cytoplasmic pressure, and explains how regulation of each property impacts the shapes of dividing cells in tissues. ER -