RT Journal Article
SR Electronic
T1 UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 081257
DO 10.1101/081257
A1 Robert C. Edgar
YR 2016
UL http://biorxiv.org/content/early/2016/10/15/081257.abstract
AB Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2.