TY - JOUR T1 - UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing JF - bioRxiv DO - 10.1101/081257 SP - 081257 AU - Robert C. Edgar Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/15/081257.abstract N2 - Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2. ER -