%0 Journal Article %A Robert C. Edgar %T UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing %D 2016 %R 10.1101/081257 %J bioRxiv %P 081257 %X Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2. %U https://www.biorxiv.org/content/biorxiv/early/2016/10/15/081257.full.pdf