TY - JOUR T1 - Single Cell Phenotyping Reveals Heterogeneity among Haematopoietic Stem Cells Following Infection JF - bioRxiv DO - 10.1101/080416 SP - 080416 AU - Adam L MacLean AU - Maia A Smith AU - Juliane Liepe AU - Aaron Sim AU - Reema Khorshed AU - Nico Scherf AU - Axel Krinner AU - Ingo Roeder AU - Cristina Lo Celso AU - Michael PH Stumpf Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/12/080416.abstract N2 - The haematopoietic stem cell (HSC) niche provides essential micro-environmental cues for the production and maintenance of HSCs within the bone marrow. During inflammation, haematopoietic dynamics are perturbed, but it is not known whether changes to the HSC-niche interaction occur as a result. We visualise HSCs directly in vivo, enabling detailed analysis of the 3D niche dynamics and migration patterns in murine bone marrow following Trichinella spiralis infection. Spatial statistical analysis of these HSC trajectories reveals two distinct modes of HSC behaviour: (i) a preference for revisiting previously explored space, and (ii) a preference for exploring new space. Whereas HSCs from control donors predominantly follow pattern (i), those from infected mice follow both. Using detailed computational analyses of cell migration tracks and life-history theory, we show that the increased motility of HSCs following infection can, perhaps counterintuitively, enable mice to cope better in deteriorating HSC-niche micro-environments following infection. ER -