RT Journal Article SR Electronic T1 Comparative transcriptome analysis by deep RNA sequencing at early stage of skin pigmentation in goats (Capra hircus) JF bioRxiv FD Cold Spring Harbor Laboratory SP 079871 DO 10.1101/079871 A1 Hangxing Ren A1 Gaofu Wang A1 Jing Jiang A1 Liangjia Liu A1 Nianfu Li A1 Jie Li A1 Lin Fu A1 Haiyan Zhang A1 Risu Na A1 Yongfu Huang A1 Li Zhang A1 Lei Chen A1 Yong Huang A1 Peng Zhou YR 2016 UL http://biorxiv.org/content/early/2016/10/09/079871.abstract AB Although specific genes have been found to be associated with skin pigmentation, the global gene expression profile for the early stage of skin pigmentation and development in mammals is still not well understood. Here we reported a rare natural group of goat (Youzhou dark goat) featuring the dark skin of body including the visible mucous membranes, which may be an exclusive kind of large mammalian species with this special phenotype so far. In the present study, we characterized the 100-day-old fetal skin transcriptome in hyperpigmented (dark-skinned) and wild-type (white-skinned) goats using deep RNA-sequencing. A total of 923,013,870 raw reads from 6 libraries were obtained, and a large number of alternative splicing events were identified in the transcriptome of fetal skin, including the well-known melanogenic genes ASIP, TYRP1, and DCT, which were differentially expressed in the skin between the dark-skinned and white-skinned goats. Further analysis demonstrated that differential genes including ASIP, TYRP1, DCT, WNT2, RAB27A, FZD4, and CREB3L1 were significantly overrepresented in the melanogenesis pathway and several biological process associated with pigmentation. On the other hand, we identified 1616 novel transcripts in goat skin based on the characteristics of their expression level and gene composition. These novel transcripts may represent two distinct groups of nucleic acid molecules. Our findings contribute to the understanding of the characteristics of global gene expression at early stages of skin pigmentation and development, as well as describe an animal model for human diseases associated with pigmentation.