TY - JOUR T1 - Portable and Error-Free DNA-Based Data Storage JF - bioRxiv DO - 10.1101/079442 SP - 079442 AU - S. M. Hossein Tabatabaei Yazdi AU - Ryan Gabrys AU - Olgica Milenkovic Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/10/05/079442.abstract N2 - DNA-based storage is an emerging nonvolatile memory technology of potentially unprecedented density, durability, and replication efficiency1,2,3,4,5,6. The basic system implementation steps include synthesizing DNA strings that contain user information and subsequently reading them via high-throughput sequencing technologies. All existing architectures enable reading and writing, while some also allow for editing and random access3 and elementary sequencing error correction3,4. However, none of the current architectures offers error-free and random-access readouts from a portable device. Here we show through experimental and theoretical verification that such a platform may be easily implemented in practice. The gist of the approach is to design an integrated pipeline that encodes arbitrary data so as to avoid costly synthesis and sequencing errors, enables random access through addressing, and leverages efficient portable nanopore sequencing via new anchored iterative alignment techniques and insertion/deletion error-correcting codes. Our work represents the only known random access DNA-based data storage system that uses error-prone MinION sequencers and produces error-free readouts with the highest reported information rate and density. ER -