RT Journal Article
SR Electronic
T1 Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 042028
DO 10.1101/042028
A1 Ruairidh J. H. Sawers
A1 Simon F. Svane
A1 Clement Quan
A1 Mette Grønlund
A1 Barbara Wozniak
A1 Eliécer González-Muñoz
A1 Ricardo A. Chávez Montes
A1 Ivan Baxter
A1 Jerome Goudet
A1 Iver Jakobsen
A1 Uta Paszkowski
YR 2016
UL http://biorxiv.org/content/early/2016/10/03/042028.abstract
AB Plant interactions with arbuscular mycorrhizal fungi have long excited interest for their potential to promote more efficient use of mineral resources in agricultural production. Their use, however, remains limited, in part because of a lack of understanding of the factors that determine symbiotic outcome. In this work, variation in response to arbuscular mycorrhizal colonization was characterized a panel of genetically diverse maize inbred lines.The parents of the maize Nested Association Mapping population were evaluated, with and without colonization, in early vegetative stages. Subsequently, six lines with contrasting phenotypes were selected for further characterization, including quantification of fungal colonization, mycorrhiza-mediated phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters.The relative growth of lines changed between non-inoculated and inoculated plants, indicative of variation in host capacity to profit from symbiosis. Patterns of Pht1 transcript accumulation varied among lines, and were correlated with outcome.Larger growth responses were correlated with more extensive development of root-external hyphae, increased accumulation of specific Pht1 transcripts and a high level of mycorrhiza-mediated phosphorus uptake. The data suggest that host genetic factors influence fungal growth strategy with subsequent impact on plant biomass production.