TY - JOUR T1 - “In-silico studies of facilitated VEGF(s) – VEGFR(s) bindings for assessment of Lysine as an indirect Low-Mol-Wt angiogen: Experimental validation of a potential synthetic Low-Mol-Wt angiogen” JF - bioRxiv DO - 10.1101/077677 SP - 077677 AU - Priyanshu Verma AU - Aritri Bir AU - Anindita Banerjee AU - Joy Basu AU - Sujoy Kar AU - Sujoy Kumar Samanta AU - Debatosh Datta Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/09/26/077677.abstract N2 - In-vivo angiogenesis process is highly conserved and is mediated through a family of peptides having VEGF-A as the lead member. A respective receptor family comprising of members VEGFR-1, 2, 3 gets expressed on the endothelial cell membrane of the vascular bed in ischemic zone along with parallel expressions of VEGF-A, B, C, D and PlGF. Degree of ischaemia is the main regulator of these coupled expressions of angiogenic peptides/factors (AFs) and respective receptor(s) for a paracrine angiogenic process to take place. Physiological angiogenesis in intrauterine growth phase is the lead process in foetal growth, organogenesis and cellular specialization. Post birth and with aging, this process gets gradually inefficient and slow. In the present in-silico study, all angiogenic factors and receptor species are examined as for their binding stability in basal unaided condition and in presence of a possible Low-Mol-Wt linkage molecule–Lysine. Also a Lysine analogue 1,6-diaminohexanoic acid has been examined for its angiogenic potential both in dry docking experiment and in cell culture assay. ER -