PT - JOURNAL ARTICLE AU - Katherine F Donovan AU - Mudra Hegde AU - Meagan Sullender AU - Emma W Vaimberg AU - Cory M Johannessen AU - David E Root AU - John G Doench TI - Mapping protein function with CRISPR/Cas9-mediated mutagenesis AID - 10.1101/076919 DP - 2016 Jan 01 TA - bioRxiv PG - 076919 4099 - http://biorxiv.org/content/early/2016/09/23/076919.short 4100 - http://biorxiv.org/content/early/2016/09/23/076919.full AB - CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. We describe a CRISPR/Cas9-mediated approach to mutagenesis, exploiting the allelic diversity generated by error-prone non-homologous end-joining (NHEJ) to identify gain-of-function alleles of the MAPK signaling pathway genes MEK1 and BRAF. These results illustrate a scalable technique to easily generate cell populations containing thousands of endogenous allelic variants of any gene or genes to map variant functions.