TY - JOUR T1 - Frequent and Transient Acquisition of Pluripotency During Somatic Cell Trans-Differentiation with iPSC Reprogramming Factors JF - bioRxiv DO - 10.1101/008284 SP - 008284 AU - Itay Maza AU - Inbal Caspi AU - Sergey Viukov AU - Yoach Rais AU - Asaf Zviran AU - Shay Geula AU - Vladislav Krupalnik AU - Mirie Zerbib AU - Rada Massarwa AU - Noa Novershtern AU - Jacob H. Hanna Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/08/23/008284.abstract N2 - Recent reports have proposed a new paradigm for obtaining mature somatic cell types from fibroblasts without going through a pluripotent state, by briefly expressing canonical iPSC reprogramming factors Oct4, Sox2, Klf4, c-Myc (abbreviated as OSKM) in cells expanded in lineage differentiation promoting conditions. Here we apply genetic lineage tracing for endogenous Nanog locus and X chromosome reactivation during OSKM induced trans-differentiation, as these molecular events mark final stages for acquisition of induced pluripotency. Remarkably, the majority of reprogrammed cardiomyocytes or neural stem cells derived from mouse fibroblasts via OSKM mediated trans-differentiation (∼>90%), are attained after transient acquisition of pluripotency, and followed by rapid differentiation. Our findings underscore a molecular and functional coupling between inducing pluripotency and obtaining “trans-differentiated” somatic cells via OSKM induction, and have implications on defining molecular trajectories assumed during different cell reprogramming methods. ER -